Journal: Journal of Functional Foods

Article Title: Rutin mitigates hepatic fibrogenesis and inflammation through targeting TLR4 and P2X7 receptor signaling pathway in vitro and in vivo

doi: 10.1016/j.jff.2019.103700

Figure Lengend Snippet: Fig. 3. Rutin mediated TLR4 and P2X7r signaling pathways reverses the activation of HSCs (A) Representative western blotting analysis of TLR4, TLR2, TLR3, CD14, and MyD88. (B) Representative western blotting analysis of P2X7r and ASC. Representative RT-PCR analysis for expressions of P2X7r. (C) Representative western blotting analysis of IRAK1, IRAK4, TRAF-1, and IRF-3. Densitometric values were normalized against GAPDH. (D) Immunofluorescence staining of TLR2, TLR3, TLR4, P2X7r, and IRAK4 present in 400× magnification. *P < 0.05, **P < 0.01, ***P < 0.001 vs TGF-β group, ns, no significance.

Article Snippet: Primay antibodies of MyD88 (D80F5), IRAK-1 (cs-4504) and IRAK-4 (cs-4363) were purchased from Cell Signaling (Massachusetts, USA).

Techniques: Protein-Protein interactions, Activation Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Staining

Journal: Journal of Functional Foods

Article Title: Rutin mitigates hepatic fibrogenesis and inflammation through targeting TLR4 and P2X7 receptor signaling pathway in vitro and in vivo

doi: 10.1016/j.jff.2019.103700

Figure Lengend Snippet: Fig. 5. Rutin regulates TLR4 and P2X7r signaling in rat primary hepatocytes. (A) MTT assay on cell viability of rat primary hepatocytes with rutin treatment. (B) Representative western blotting analysis of TLR4, CD14, and MyD88. (C) Representative western blotting analysis of IRAK1, IRAK4, TRAF-1, and IRF-3. (D) Representative western blotting analysis of NLRP3, P2X7r, ASC, and IL-1α. Densitometric values were normalized against GAPDH. #P < 0.05, ###P < 0.001 vs nromal group; *P < 0.05, **P < 0.01, ***P < 0.001 vs TGF-β group, ns, no significance.

Article Snippet: Primay antibodies of MyD88 (D80F5), IRAK-1 (cs-4504) and IRAK-4 (cs-4363) were purchased from Cell Signaling (Massachusetts, USA).

Techniques: MTT Assay, Western Blot

Journal: Journal of Functional Foods

Article Title: Rutin mitigates hepatic fibrogenesis and inflammation through targeting TLR4 and P2X7 receptor signaling pathway in vitro and in vivo

doi: 10.1016/j.jff.2019.103700

Figure Lengend Snippet: Fig. 8. Rutin ameliorates hepatic fibrosis by regulating TLR4 signaling in TAA-induced mice. (A) Representative western blotting analysis of TLR2, TLR3, TLR4, CD14, and MyD88. Densitometric values were normalized against GAPDH. (B) Immunofluorescence staining of TLR4 present in 600× magnification. (C) Representative western blotting analysis of IRAK1, IRAK4, TRAF-1, and IRF-3. Densitometric values were normalized against GAPDH. (D) Representative RT-PCR analysis for expressions of TLR4 and CD14. Densitometric values were normalized against GAPDH. (E) Immunofluorescence staining of IRAK1 and IRAK4 present in 200× magnification. ###P < 0.001 vs normal group, *P < 0.05, **P < 0.01, ***P < 0.001 vs TAA group, ns, no significance.

Article Snippet: Primay antibodies of MyD88 (D80F5), IRAK-1 (cs-4504) and IRAK-4 (cs-4363) were purchased from Cell Signaling (Massachusetts, USA).

Techniques: Western Blot, Staining, Reverse Transcription Polymerase Chain Reaction

Journal: Biochemical and biophysical research communications

Article Title: Pulsed ultrasound prevents lipopolysaccharide-induced muscle atrophy through inhibiting p38 MAPK phosphorylation in C2C12 myotubes.

doi: 10.1016/j.bbrc.2021.07.039

Figure Lengend Snippet: Fig. 3. The effect of the pulsed ultrasound on IRAK4 and p38 MAPK with or without LPS administration. Phosphorylation and total protein level of IRAK4 (A) and p38 MAPK (B) were measured in this research. Values were calculated as fold changes relative to the Cont group and represented as mean ± SEM. * and y indicate a significant difference (P < 0.05) from LPS treatment and pulsed ultrasound irradiation respectively.

Article Snippet: Membranes were incubated with antibodies against Myosin Heavy Chain (1:1000 in TBST,14-6503, eBioscience), phospho-IRAK4 (1:1000 in TBST, #11927, Cell Signaling, Danvers, Mass, USA), IRAK4 (1:1000 in PBST, #4363, Cell Signaling), phospho-p38 MAPK (1:1000 in TBST, #4511, Cell Signaling), p38 MAPK (1:1000 in PBST, #9212, Cell Signaling), phospho-FAK (1:1000 in TBST, #3283, Cell Signaling), FAK (1:1000 in PBST, #3285, Cell Signaling), or GAPDH (1:1000 in PBST, Y3322, BioChain Institute, Newark, CA, USA) at 4 C overnight.

Techniques: Phospho-proteomics, Irradiation